Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
AssigneesThe Board Institute, Inc., Massachusetts Institute of Technology
InventorsFeng ZHANG, Fei RAN
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Abstract
The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.
Claims30
A modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) enzyme wherein the enzyme is a Cas9 ortholog of a genus belonging to the group consisting of Corynebacter, Sutterella, Legionella, Treponemna, Filifactor, Eubacterium, Streptococcus, Lactobacillus, Mycoplasma, Bacteroides, Flaviivola, Flavobacterium, Sphaerochaeta, Azospirillum, Gluconacetobacter, Neisseria, Roseburia, Parvibaculum, Staphylococcus, Nitratifractor, Mycoplasma and Campylobacter and wherein the CRISPR enzyme comprises a chimeric CRISPR enzyme.
Dependencies are inferred from the claim text.