Crispr-based genome modification and regulation
AssigneeSigma-Aldrich Co.
InventorsFuqiang Chen, Gregory Davis
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Abstract
The present invention provides methods for modifying chromosomal sequences. In particular, methods are provided for using RNA-guided endonucleases or modified RNA-guided endonucleases to modify targeted chromosomal sequences.
Claims21
-9. (canceled)
A fusion protein comprising a CRISPR/Cas-like protein or fragment thereof and a cleavage domain.
A method for modifying a chromosomal sequence in a cell or embryo, the method comprising introducing into the cell or embryo (a) at least one fusion protein or a nucleic acid encoding the fusion protein, the fusion protein comprising a CRISPR/Cas-like protein or fragment thereof and a cleavage domain, and (b) at least two guiding RNAs or DNA encoding the guiding RNAs, wherein the guiding RNAs guide the CRISPR/Cas-like protein of the fusion protein to two different targeted sites in the chromosomal sequences such that the fusion protein dimerizes and the cleavage domain of the fusion protein introduces a double stranded break into the chromosomal sequence that is repaired by a DNA repair process such that the chromosomal sequence is modified.
Dependencies are inferred from the claim text.