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US 2014/0273229 A1Published application
Intracellular delivery and transfection methods and devices
AssigneeOpencell Technologies, Inc.
InventorsJohn Mark Meacham, Kiran Durvasula, Andrei G. Fedorov, Fahrettin Levent Degertekin, Akash Mehta
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Abstract
Embodiments of the present disclosure provide a multistage procedure for treatment of biological samples (e.g., living cells with membranes, and the like) with a substance (e.g., a drug, DNA, RNA, plasmids, and other biomolecules or materials) to achieve more efficacious intracellular delivery and transfection.
Claims17
A method of delivering a substance into a living cell having a cell membrane, comprising:
- a) permeabilization of the cell membrane in a suspension, and
- b) insertion of a substance into the cell interior through pores formed during permeabilization of the membrane.
Dependencies are inferred from the claim text.