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US 2014/0273225 A1Published application
Nucleosome-excluding sequences (nes) as a dna spacer in vectors results in prolonged transgene expression in eukaryotic cells
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJiamiao Lu, Mark A. Kay, Andrew Fire, Lia E. Gracey Maniar
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Abstract
The silencing effect of a spacer sequence, for example a bacterial backbone sequence in a plasmid or other episomal vector, on transgene expression is reversed by engineering of the spacer to include nucleosome exclusion sequences.
Claims19
A method of reducing silencing of a transgene present in a vector comprising a gene silencing sequence;
- the method comprising:
- modifying the sequence of the gene silencing sequence by introduction of one or more nucleosome-excluding sequences (NES).
Dependencies are inferred from the claim text.