Micro-device for culturing cells, method for manufacturing same, and method for culturing cells using the micro-device for culturing cells
AssigneeUnist Academy-Industry Research Corporation
InventorsYoon-Kyoung Cho, Hyundoo Hwang
Life of this patent
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Abstract
Disclosed is micro-device for culturing cells comprising: a plurality of fluid paths through which fluid moves; and at least one inlet port for injecting fluid to the fluid paths, said fluid paths communicating with each other and being different in height from each other. In the cell culture device having a plurality of fluid paths, cells can be cultured by introducing a polymeric material to at least one fluid path having a relatively low height; solidifying the polymeric material to form a 3-dimensional scaffold; and injecting fluid for cell culture to a fluid path in contact with the 3-dimensional scaffold.
Claims21
A micro-device for culturing cells, comprising:
- a plurality of fluid paths through which fluid moves;
- and
- at least one inlet port for injecting fluid to the fluid paths, said fluid paths communicating with each other and being different in height from each other.
A method for manufacturing micro-device for culturing cells, comprising:
- forming a first microstructure having the same height as a first fluid path on an area of a flat plate, said area corresponding to a sum of the first fluid path and a second fluid path lower in height than the first fluid path;
- forming a second microstructure having a height corresponding to a difference between the heights of the first fluid path and the second fluid path on the first microstructure;
- applying a liquid material for forming the fluid paths to the first microstructure and the second microstructure, followed by solidifying the liquid material;
- overlying the solidified material to a substrate to form the first fluid path and the second fluid path, with a connection therebetween;
- introducing a polymeric material to the second fluid path to form a 3-dimensional scaffold;
- and
- injecting fluid to the first fluid path.
A method for culturing cells using the micro-device for culturing cells having a plurality of fluid paths, comprising:
- introducing a polymeric material to at least one fluid path having a relatively low height;
- solidifying the polymeric material to form a 3-dimensional scaffold;
- and
- injecting fluid for cell culture to a fluid path in contact with the 3-dimensional scaffold.
Dependencies are inferred from the claim text.