Back to results
US 2014/0273221 A1Published application
Substrates for generation of controlled human pluripotent stem cell colony size and shape
AssigneeWisconsin Alumni Research Foundation
InventorsJustin T. Koepsel, William L. Murphy, Sheeny K.L. Levengood, Stefan Zorn
Life of this patent
Estimating the term…
Abstract
Methods of cell culture using patterned SAM arrays are disclosed. Advantageously, the disclosed methods use SAM arrays presenting adhesion peptides to grow confluent monolayers that can invaginate to form an embryoid body.
Claims19
A method of controlling the formation of a cell culture aggregate, the method comprising:
- culturing a cell on an array spot of a self-assembled monolayer array for a sufficient time to form a confluent monolayer of cells;
- and detaching the confluent monolayer of cells.
A method of preparing a cell aggregate of a uniform size, the method comprising:
- culturing a cell on an array spot of a self-assembled monolayer array spot of a specified diameter for a sufficient time to form a confluent monolayer of cells;
- detaching the confluent monolayer of cells;
- and
- collecting the confluent monolayer of cells.
1 more element
A method of preparing a cell aggregate of a specified shape, the method comprising:
- culturing a cell on a self-assembled monolayer array spot of a specified shape for a sufficient time to form a confluent monolayer of cells;
- detaching the confluent monolayer of cells;
- and
- collecting the confluent monolayer of cells.
1 more element
Dependencies are inferred from the claim text.