Label-free identification of stem cell-differentiated cells
AssigneesThe Regents of the University of California, Lawrence Livermore National Security, LLC
InventorsJames W. Chan, Deborah Lieu
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Abstract
Methods are disclosed for isolation of a stem-cell derived differentiated cell, which method entails illuminating an incident light onto a plurality of stem-cell derived cells that comprise at least an undifferentiated cell and a differentiated cell possessing a noncentrosymmetric structure, wherein the differentiated cell generates second-harmonic light from the incident light; and isolating the differentiated cell identified by the second-harmonic light. Devices for carrying out the methods are also provided.
Claims25
A method for isolation of a stem-cell derived differentiated cell, comprising:
- illuminating an incident light onto a plurality of stem-cell derived cells that comprise at least an undifferentiated cell and a differentiated cell possessing a noncentrosymmetric structure, wherein the differentiated cell generates second-harmonic light from the incident light;
- and
- isolating the differentiated cell identified by the second-harmonic light.
A method of isolating cells comprising:
- passing a plurality of cells through a device comprising (a) a laser light source to cast an incident light on the cells and (b) a sensor configured to collect or detect second-harmonic light generated from the incident light, wherein the cells comprise at least an undifferentiated stem cell and a differentiated cell comprising a noncentrosymmetric structure;
- identifying the differentiated cell by the second-harmonic light generated on the cell;
- and
- collecting the differentiated cell.
A method of distinguishing two cells of different differentiation stages, comprising:
- culturing one or more stem cells under conditions to allow the stem cells to differentiate to two differentiated cells at different differentiation stages, at least one of which comprises a noncentrosymmetric structure;
- illuminating an incident light on the differentiated cells;
- and
- distinguishing the two differentiated cells by examining second-harmonic light generated on at least one of the two differentiated cells.
A system comprising (a) a laser light source to produce an incident light, (b) a sensor configured to collect or detect second-harmonic light generated from the incident light, and (c) a plurality of cells comprising at least an undifferentiated stem cell and a differentiated cell comprising a noncentrosymmetric structure.
Dependencies are inferred from the claim text.