Cartridge for nucleic acid amplification reaction
AssigneeSeiko Epson Corporation
InventorsYuji Saito, Fumio Takagi
Life of this patent
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Abstract
A cartridge for nucleic acid amplification reaction includes a tube that has a first plug, a second plug formed of a first washing solution which washes nucleic acid-binding solid-phase carriers having bound to a nucleic acid, a third plug, a fourth plug formed of an eluate which causes the nucleic acid to be eluted from the nucleic acid-binding solid-phase carriers having bound to the nucleic acid, and a fifth plug in this order in the inside of the tube; a container for nucleic acid amplification reaction that is in communication with the side of the fifth plug of the tube and contains oil; and a plunger that pushes liquid to the container for nucleic acid amplification reaction out of the side of the fifth plug of the tube.
Claims20
A cartridge for nucleic acid amplification reaction comprising:
- a tube that has a first plug formed of a first oil, a second plug formed of a first washing solution which undergoes phase separation when being mixed with oil and washes nucleic acid-binding solid-phase carriers having bound to a nucleic acid, a third plug formed of a second oil, a fourth plug formed of an eluate which undergoes phase separation when being mixed with oil and causes the nucleic acid to be eluted from the nucleic acid-binding solid-phase carriers having bound to the nucleic acid, and a fifth plug formed of a third oil in this order in the inside of the tube;
- a container for nucleic acid amplification reaction that is in communication with the side of the fifth plug of the tube and contains oil;
- and
- a pushing unit that is mounted on an opening portion of the side of the first plug of the tube and pushes liquid to the container for nucleic acid amplification reaction out of the side of the fifth plug of the tube.
Dependencies are inferred from the claim text.