Point of care sensor systems

An electrochemical sensor assembly comprising:

• a cartridge comprising a sample inlet port, one or more solid reagent compartments, one or more liquid reagent compartments, and a sensor well including two or sensors configured to detect biomolecules in the sample;
• and
• a reader configured to receive the cartridge, the reader configured to provide a plurality of pneumatic inputs to the cartridge and receive electric signals from the cartridge indicating detection information of two or more target analytes in a sample.

-23. (canceled)

A reader configured to receive a cartridge, comprising:

• a pneumatic assembly configured to supply vacuum to the cartridge, at a set level Pv and pressure at a set level Pp, wherein Pp is greater than Pv;
• and
• a detection assembly configured to receive electrical signal information from the cartridge.

(canceled)

A cartridge for sensing first and second target analytes in a biological sample, comprising:

• a first working electrode coated with one or more enzymes configured to react with a first target analyte to directly or indirectly produce a first electrochemical signal;
• a second working electrode having capture species attached thereto, the capture species configured to capture the second target analyte to directly or indirectly produce a second electrochemical signal,
• wherein the cartridge is a single sample, single use, disposable cartridge.

(canceled)

(canceled)

34-35. (canceled)