Propagating an organism and related methods and compositions
AssigneePOET Research, Inc.
InventorNeelakantam V. Narendranath
Life of this patent
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Abstract
The present invention is related to methods of propagating one or more organisms by using a carbon source that includes xylose (e.g., xylose syrup from pretreating lignocellulosic feedstock) and/or a nutrient source that includes a stillage component (e.g., thin stillage derived from a corn-to-ethanol process) in the propagation medium. The organisms include those that can convert one or more monosaccharides into an alcohol via fermentation, such as yeast. The present invention is also directed to related compositions.
Claims21
A method of propagating an organism that can convert one or more monosaccharides into a biochemical, the method comprising:
- providing a first cell mass of the organism;
- providing a carbon source that can support growth of the first cell mass of the organism, wherein the carbon source comprises at least xylose;
- providing a nutrient source that can support growth of the first cell mass of the organism, wherein the nutrient source comprises a stillage component;
- combining at least the carbon source and the nutrient source to form a medium for propagating the organism;
- and
- combining the first cell mass of the organism with the carbon source and the nutrient source to propagate the first cell mass of the organism for a time period to form a second cell mass of the organism.
A method of propagating an organism that can convert one or more monosaccharides into a biochemical, the method comprising:
- providing a first cell mass of the organism;
- providing a carbon source that can support growth of the first cell mass of the organism, wherein the carbon source comprises one or more monosaccharides;
- providing a nutrient source that can support growth of the first cell mass of the organism, wherein the nutrient source comprises a stillage component;
- combining at least the carbon source and the nutrient source to form a medium for propagating the organism, wherein the first cell mass is present in an amount less than 1.0 gram of organisms per liter of medium;
- and
- combining the first cell mass of the organism with the carbon source and the nutrient source to propagate the first cell mass of the organism for a time period to form a second cell mass of the organism.
A composition comprising:
- a first cell mass of an organism that can convert one or more monosaccharides into a biochemical, wherein the organism is present in an amount of less than 1.0 gram of organisms per liter of the composition;
- xylose in an amount in the range of from 0.1 to 10 percent by weight of the composition;
- and
- a stillage component in an amount in the range of from 5 to 35 grams solids per liter of the composition.
Dependencies are inferred from the claim text.