Non-co2 evolving metabolic pathway for chemical production
AssigneeThe Regents of the University of California
InventorsJames C. Liao, Igor Bogorad
Life of this patent
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Abstract
Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a suitable carbon source. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.
Claims46
A recombinant microorganism comprising a non-CO2 evolving metabolic pathway for the synthesis of acetyl phosphate with improved carbon yield beyond 1:2 molar ratio (fructose 6-phosphate:Acetyl phosphate) from a carbon substrate using a pathway comprising an enzyme having fructose-6-phosphoketolase (Fpk) activity and/or xylulose-5-phosphoketolase (Xpk) activity.
A recombinant microorganism comprising a non-CO2-evolving pathway that comprises synthesizing acetyl phosphate using a recombinant metabolic pathway that metabolizes methanol, methane, formate, formaldehyde, CO2, CO, a carbohydrate having the general formula CnH2nOn wherein n=3 to 7, or a sugar phosphate metabolite, with improved carbon yield beyond those obtained by pathways that involve pyruvate decarboxylation.
A recombinant microorganism comprising a pathway that produces acetyl-phosphate through carbon rearrangement of E4P and metabolism of a carbon source selected from methanol, methane, formate, formaldehyde, CO2, CO, a carbohydrate (CnH2nOn, n=3-7) or a sugar phosphate.
A recombinant microorganism expressing enzymes that catalyze the conversion described in (i)-(ix), wherein at least one enzyme or the regulation of at least one enzyme that performs a conversion described in (i)-(ix) is heterologous to the microorganism:
- (i) the production of acetyl-phosphate and erythrose-4-phosphate (E4P) from fructose-6-phosphate and/or the production of acetyl-phosphate and glyceraldehyde 3-phosphate (G3P) from xylulose 5-phosphate;
- (ii) the conversion of fructose-6-phosphate and E4P to sedoheptulose 7-phosphate (S7P) and (G3P) or the reverse thereof;
- (iii) the conversion of S7P and G3P to ribose-5-phosphate and xylulose-5-phosphate or the reverse thereof;
- (iv) the conversion of ribose-5-phosphate to ribulose-5-phosphate or the reverse thereof;
- (v) the conversion of ribulose-5-phosphate to xylulose-5-phosphate or the reverse thereof;
- (vi) the conversion of xylulose-5-phosphate and E4P to fructose-6-phosphate and glyceraldehyde-3-phosphate or the reverse thereof;
- (vii) the conversion of glyceraldehyde-3-phosphate to dihydroxyacetone phosphate or the reverse thereof;
- (viii) the conversion of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate to fructose 1,6 biphosphate or the reverse thereof;
- and
- (ix) the conversion of fructose 1,6-biphosphate to fructose-6-phosphate,
- wherein the microorganism produces acetyl-phosphate, or compounds derived from acetyl-phosphate using a carbon source selected from the group consisting of a carbohydrate having the general formula (CnH2nOn, n=3-7), a sugar-phosphate, CO2, CO, methanol, methane, formate, formaldehyde and any combination thereof.
Dependencies are inferred from the claim text.