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US 2014/0273162 A1Published application
Metabolically engineered escherichia coli for enhanced production of sialic acid
AssigneeSyracuse University
InventorsChristopher N. Boddy, Benjamin R. Lundgren
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Abstract
A metabolically engineered E. coli strain which produces sialic acid and a method of making said strain. In the engineered E. coli cells, the nanT (sialic acid transporter) and nanA (sialic acid adolase) genes are inactivated, and the neuC and neuB genes of sialic acid biosynthesis in Neisseria meningitidis group B are introduced and overexpressed in the nanT− nanA− E. coli cell. In addition, the glucosamine synthase gene, glmS, of E. coli is co-overexpressed with neuB and neuC.
Claims10
A genetically engineered microbe, comprising:
- an inactivating mutation in a first DNA molecule in the microbe, wherein the first DNA molecule encodes a sialic acid transporter;
- an inactivating mutation in a second DNA molecule in the microbe, wherein the second DNA molecule encodes a sialic acid aldolase;
- and
- a first enzyme-encoding recombinant DNA molecule, wherein the first enzyme-encoding recombinant DNA molecule encodes an enzyme selected from the group consisting of a sialic acid synthase and a UDP-N-acetylglucosamine 2-epimerase.
1 more element
Dependencies are inferred from the claim text.