Staged Bacteriophage Remediation of Target Bacteria
AssigneeDow Global Technologies LLC
InventorsElizabeth J. Summer, Mei Liu, Neil S. Summer, Douglas Baldwin
Life of this patent
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Abstract
A process for remediation of target bacteria, particularly sulfur reducing bacteria (SRB), in waters (“target water”) having a multiplicity and diverse host target bacteria by employing serial or staged bacteria culturing and lysing of dominant bacteria. Remediation of sulfur reducing bacteria (SRB) is effected by application of a series of bacteriophage isolated from the staged culturing and bacteriophage lysing of successive aliquots of waters containing a multiplicity of SRB.
Claims16
A process for control of a broad range of target bacteria comprising:
- culturing a dominant group of bacteria in a mixed bacteria solution, isolating virulent phage(s) for the dominate a bacteria, lysing dominant bacteria from a sample of the mixed bacteria solution to remove the dominant bacteria from the mix;
- culturing the next dominant strain from the solution with the dominate strain lysed, isolating a virulent phage for the next dominate bacteria, lysing it to remove the next dominate bacteria;
- and
- continuing the sequence to provide a set of bacteriophage that will lyse the dominate and sub-dominate bacteria;
- applying an effective amount of the bacteriophage so isolated in each step either as a mixture or in sequence application to waters containing a mixture of target bacteria of those found in each step of the above sequence.
A process for control of a broad range of target bacteria comprising:
- culturing a dominant group of bacteria from a diverse mixture of target bacteria, isolating virulent phage active against the dominant bacteria;
- isolation of temperate phages from these bacterial strains from the culture supernatant;
- stressing the temperate bacteria to convert into virulent phages for use in phage preparations;
- continuing the sequence until a collection of phage is assembled that will effect significant and lasting of problematic bacteria;
- and
- applying an effective amount of the phage so isolated in each step either as a mixture or in sequence application to waters containing the a mixture of target bacteria of those found in each step of the above sequence.
A composition of bacteriophage comprising bacteriophage produced by;
- culturing a dominant group of bacteria in a mixed bacteria solution, isolating virulent phage(s) for the dominate a bacteria, lysing dominant bacteria from a sample of the mixed bacteria solution to remove the dominant bacteria from the mix;
- culturing the next dominant strain from the solution with the dominate strain lysed, isolating a virulent phage for the next dominate bacteria, lysing it to remove the next dominate bacteria;
- and
- continuing the sequence to provide a set of bacteriophage that will lyse the dominate and sub-dominate bacteria.
Dependencies are inferred from the claim text.