Luciferase biosensor
AssigneePromega Corporation
InventorsFrank Fan, Martin Ken Lewis, John W. Shultz, Keith V. Wood, Braeden Butler
Life of this patent
Estimating the term…
Abstract
A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.
Claims22
An isolated polynucleotide encoding a modified beetle luciferase comprising an internal insertion of a heterologous amino acid sequence which interacts with a molecule of interest, wherein the activity of the modified beetle luciferase is altered after the molecule of interest interacts with the insertion sequence relative to the activity before the interaction, wherein the internal insertion is at a residue or region in a beetle luciferase sequence which is tolerant to modification.
A isolated polynucleotide encoding a circularly permuted modified beetle luciferase comprising an internal insertion of a heterologous amino acid sequence which interacts with a molecule of interest, wherein the activity of the modified beetle luciferase is altered after the molecule of interest interacts with the insertion sequence relative to the activity before the interaction, wherein the circular permutation is at a residue or region in a beetle luciferase sequence which is tolerant to modification.
Dependencies are inferred from the claim text.