Dual-functional hybrid glucanases
AssigneeAcademia Sinica
InventorsLie-Fen Shyur, Wei-Chun Liu
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Abstract
Described herein is a fusion polypeptide that contains (a) a first segment containing a Fibrobacter succinogenes 1,3-1,4-β-D-glucanase (Fsβ-glucanase) or a fragment thereof; (b) a second segment containing a first Thermotoga maritima 1,3-β-D-glucanase (TmLam) or a fragment thereof; and (c) an optional third segment containing a second Thermotoga maritima 1,3-β-D-glucanase (TmLam) or a fragment thereof; wherein the fusion polypeptide has a glucanase activity. Also described are a nucleic acid molecule encoding the fusion polypeptide and a method of using the polypeptide.
Claims19
A fusion polypeptide, the polypeptide comprising:
- (a) a first segment containing a Fibrobacter succinogenes 1,3-1,4-β-D-glucanase (Fsβ-glucanase) or a fragment thereof;
- (b) a second segment containing a first Thermotoga maritima 1,3-β-D-glucanase (TmLam) or a fragment thereof;
- and
- (c) an optional third segment containing a second Thermotoga maritima 1,3-β-D-glucanase (TmLam) or a fragment thereof;
- wherein the fusion polypeptide has a glucanase activity.
Dependencies are inferred from the claim text.