Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
AssigneeThe Research Foundation for The State University of New York
InventorsBhavin V. Bhayani, Byeong Cheol Min, Bandaru V. Ramarao
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Abstract
A method for processing a stream of cellulosic fines containing inorganic particles, to increase a hydrolysis yield of polysaccharide degradation enzymes, such fines in a waste stream from a recycled packaging paper mill to produce a stream of fermentable sugars, comprising treating the fines with a surfactant which selectively binds to the inorganic particles and which reduces binding to the inorganic particles by the polysaccharide degradation enzymes, and degrading polysaccharides in the waste stream with the polysaccharide degradation enzymes.
Claims17
A method for processing a stream of cellulosic fines containing inorganic particles having an affinity for polysaccharide degradative enzymes, comprising:
- adding at least one surfactant to the stream in an amount sufficient to bind to surfaces of the inorganic particles to reduce interaction with the polysaccharide degradative enzymes without inactivating the polysaccharide degradative enzymes in solution;
- adding at least one polysaccharide degradative enzyme to the solution;
- and
- maintaining the solution with the cellulosic fines, at least one surfactant, and at least one enzyme for a sufficient period of time to degrade at least a portion of the cellulosic fines into fermentable sugars.
A method for enzymatically hydrolyzing a mixed stream of cellulosic fines and inorganic particles having a competitive binding affinity for hydrolytic enzymes, comprising:
- adding at least one surfactant to the stream in an amount sufficient to reduce an affinity of the hydrolytic enzymes for the inorganic particles;
- adding at least one hydrolytic enzyme to the solution;
- and
- hydrolyzing the cellulosic fines with the hydrolytic enzyme.
A system for enzymatically hydrolyzing a mixed stream of cellulosic fines and inorganic particles having a competitive binding affinity for hydrolytic enzymes, comprising:
- a feed for adding at least one surfactant to the stream in an amount sufficient to reduce an affinity of the hydrolytic enzymes for the inorganic particles;
- a feed for adding at least one hydrolytic enzyme to the solution;
- and
- an incubator hydrolyzing the cellulosic fines with the hydrolytic enzyme.
Dependencies are inferred from the claim text.