Polysialic acid, blood group antigens and glycoprotein expression in prokaryotes
AssigneeGlycobia, Inc.
InventorsJudith H. Merritt, Adam C. Fisher, Brian S. Hamilton, Matthew P. DeLisa
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Abstract
The invention described herein generally relates to glycoengineering host cells for the production of glycoproteins for therapeutic use. Host cells are modified to express biosynthetic glycosylation pathways. Novel prokaryotic host cells are engineered to produce N-linked glycoproteins wherein the glycoproteins comprise polysialic acid or blood group antigens.
Claims26
A method for producing an oligosaccharide composition comprising:
- culturing a recombinant host cell to express one or more of the enzyme activites comprising:
- a. GalNAc transferase (EC 2.4.1.-);
- b. galactosyltransferase (EC 2.4.1.-);
- c. fucosyltransferase (EC 2.4.1.69);
- and
- d. sialyltransferase (EC 2.4.99.4, EC 2.4.99.-, EC 2.4.99.8).
A host cell produced by any of the above claims.
An oligosaccharide composition produced by any of the above claims.
A glycoprotein composition produced by any of the above claims.
A recombinant host cell comprising at least one neu activity and at least one kps activity.
Dependencies are inferred from the claim text.