Nucleic acid amplification reaction device and nucleic acid amplification method

A nucleic acid amplification method comprising:

• providing a rotary body having a mounting portion;
• mounting a cartridge to the mounting portion of the rotary body, the cartridge including:
• a tube that has a plug containing an eluate in which a nucleic acid having bound to nucleic acid-binding solid-phase carriers is eluted from the carriers;
• and
• a nucleic acid amplification reaction container that is in communication with the tube and contains oil undergoing phase separation from the eluate, on a mounting portion;
• and
• forming a temperature gradient inside the nucleic acid amplification reaction container and changing a posture of the cartridge by rotating the rotary body such that a droplet of the eluate introduced from the tube moves inside the nucleic acid amplification reaction container.

A nucleic acid amplification reaction device comprising:

• a rotary body having a mounting portion on which a cartridge is mountable, the cartridge including:
• a tube for holding a plug containing an eluate;
• and
• a nucleic acid amplification reaction container fluidly communicating with the tube;
• and
• a heater operatively associated with the nucleic acid amplification reaction container and configured to form a temperature gradient inside the nucleic acid amplification reaction container,
• wherein the rotary body is adapted to change a posture of the cartridge so that a droplet of the eluate introduced from the tube moves inside the nucleic acid amplification container.

A nucleic acid amplification reaction device comprising:

• a rotary body having a mounting portion;
• a cartridge mounted on the mounting portion of the rotary body, the cartridge including:
• a tube that has a plug containing an eluate in which a nucleic acid having bound to nucleic acid-binding solid-phase carriers is eluted from the carriers;
• and
• a nucleic acid amplification reaction container that is in communication with the tube and contains oil undergoing phase separation from the eluate;
• and
• a heater thermally associated with the nucleic acid amplification reaction container so as to form a temperature gradient inside the nucleic acid amplification reaction container,
• wherein the rotary body is configured to rotatably change a posture of the cartridge so that a droplet of the eluate introduced from the tube moves inside the nucleic acid amplification container.