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US 2014/0273090 A1Published application
Methods and Systems for Peptide/Protein Amplification
AssigneeArizona Board of Regents, a body corporate of the State of Arizona, acting for and on behalf of
InventorRolf U. Halden
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Abstract
An amino acid chain of interest is selected or captured for amplification. The amino acid chain of interest including at least one amino acid sequence is denatured and read. The read amino acid sequence is transcribed to synthesize a mRNA equivalent. The amino acid chain of interest is then amplified using a biological system.
Claims24
A peptide/protein amplification reaction (PAR) method comprising:
- selecting or capturing an amino acid chain of interest for amplification;
- denaturing the amino acid chain of interest including at least one amino acid sequence;
- reading the at least one amino acid sequence and any protein modifications present;
- synthesizing at least one mRNA template, mRNA equivalent, or cDNA template complementary to the read amino acid sequence;
- and
- translating the mRNA, mRNA equivalent, or cDNA to obtain an amplified amino acid chain of interest using a biological system.
3 more elements
A peptide/protein amplification reaction (PAR) method comprising:
- selecting or capturing an amino acid chain of interest for amplification, wherein the amino acid chain of interest comprises a peptide or protein;
- denaturing the amino acid chain of interest including at least one amino acid sequence;
- reading the at least one amino acid sequence;
- transcribing the read amino acid sequence to synthesize at least one mRNA template, mRNA equivalent, or cDNA using oligonucleotide synthesis;
- increasing the number of mRNA templates, mRNA equivalents, or cDNA equivalent templates;
- and
- translating the mRNA, mRNA equivalent, or cDNA of interest to obtain a high copy number of the target amino acid sequence using a biological system.
4 more elements
Dependencies are inferred from the claim text.