Method for processing and embedding tissue

A method for treating a tissue sample, comprising

• providing a tissue cassette, comprising:
• a retaining member having an first tissue engaging surface and at least one biasing element, the first tissue engaging surface being moveably attached to the retaining member by said biasing element, and
• a base comprising a second tissue engaging surface and configured to engage the retaining member to form an interior area with the upper and second tissue engaging surfaces facing each other;
• placing the at least one tissue sample on one of the first tissue engaging surface and the second tissue engaging surface;
• attaching the base and the retaining member to cause the biasing element to urge first tissue engaging surface towards the second tissue engaging surface, such that the tissue sample is held between the tissue engaging surfaces;
• and
• processing the tissue sample in the tissue cassette with one or more solvents.

A method for treating a tissue sample, comprising

• placing at least one tissue sample on a cassette having a base and a retaining member,
• processing the tissue sample in the tissue cassette with one or more solvents,
• embedding the tissue sample in paraffin to form a portion of paraffin in which the tissue sample is embedded in the tissue cassette, and
• slicing the tissue sample which is embedded in the paraffin into layers to create a tissue section,
• wherein in the processing, embedding, and slicing steps are preformed while the tissue sample is attached to at least one of the base and the retaining member.

A method for treating a tissue sample, comprising

• providing a tissue cassette comprising:
• a retaining member having a first tissue engaging surface;
• a base having a second tissue engaging surface;
• and
• at least one biasing element,
• at least one of the first tissue engaging surface and the second tissue engaging surface is moveably attached to at least one of the retaining member and the base by said biasing element, and
• the base and the retaining member are configured to engage each other to form an interior area with the first and second tissue engaging surfaces facing each other,
• attaching the base and the retaining member to cause the biasing element to urge first tissue engaging surface towards the second tissue engaging surface, such that the tissue sample is held between the tissue engaging surfaces;
• and
• processing the tissue sample in the tissue cassette with one or more solvents.