Method and composition for staining and processing a urine sample
AssigneeIris International, Inc.
InventorJack Cremins
Life of this patent
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Abstract
The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.
Claims13
A particle contrast agent composition for staining particles of a urological fluid sample for imaging in an automated particle analysis system comprising:
- Crystal Violet present in amounts sufficient to result in concentrations between 50 μM and 500 μM under staining conditions;
- and
- a permeabilizing agent selected from the group consisting of 5 PD Lytic and saponin.
A method of treating particles of a urological fluid sample for imaging using in an automated particle analysis system comprising:
- combining a particle contrast agent composition and the urological fluid sample into a sample mixture resulting in a final concentration of the particle contrast agent composition by weight of the sample mixture between about 1% and about 20%;
- and
- incubating the sample mixture at a temperature above 20° Celsius for fewer than 90 seconds;
- wherein the particle contrast agent composition comprises:
- Crystal Violet present in amounts sufficient to result in concentrations between 50 μM and 500 μM under staining conditions;
- and
- 5 PD Lytic.
Dependencies are inferred from the claim text.