Noninvasive method for measuring antimicrobial peptides from skin as an objective measurement of natural protection from microbes

Abstract

A noninvasive method for measuring skin health in a subject comprising collecting an epithelial cell/skin cell sample from the subject; detecting a level of one or more antimicrobial peptide biomarkers in the epithelial cell sample/skin cell sample; diagnosing the subject as having a defense mechanism due to microbial attack on skin or a measure of the system being in balance with environment homeostasis based on the level of one or more antimicrobial peptide biomarkers selected from the group consisting of Histone H2B type 1-M, Histone H2A, Histone H4, Protein S100-A7, Protein S100-A8, Protein S100-A9, Cathepsin G, Neutrophil defensin 3 (Defensin, alpha 3) (HNP-3), Dermcidin, Ribonuclease 7 (RNase 7), human beta-defensin -2 (nBD-2) and Beta-defensin 103 (hBD-3) and listed in Table 1 wherein the AMP families include bactericidal permeability-increasing protein (BPI), Defensin, Histone, Pore-forming toxin, S100 protein, Cytotoxin, Serine Protease 51, Dual Oxidase, transcription regulation, and mixtures thereof. Further, a noninvasive method for evaluating the efficacy of products for skin health.

Dependencies are inferred from the claim text.