Methods of cell culture
AssigneeMomenta Pharmaceuticals, Inc.
InventorsHolly Prentice, Rasheed Tijani, Brett Belongia
Life of this patent
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Abstract
Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described.
Claims31
A method of producing a recombinant protein preparation having a target value of one or more of high mannose glycans and fucosylated glycans, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising DMSO under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has the target value of the one or more of high mannose glycans and fucosylated glycans.
A method of producing a recombinant protein preparation, the method comprising:
- (a) providing a target value of one or more of high mannose glycans and fucosylated glycans;
- (b) providing a cell genetically engineered to express a recombinant protein;
- (c) culturing the cell in a culture medium comprising DMSO under conditions in which the cell expresses the recombinant protein;
- (d) harvesting a preparation of the recombinant protein produced by the cell;
- and
- (e) formulating the preparation into a drug product if the preparation meets the target value of the one or more of high mannose glycans and fucosylated glycans.
A method of increasing a level of one or more of high mannose glycans, sialylated glycans, and 3,3,1,0,0 glycans in a recombinant protein preparation, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising DMSO under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has an increased level of one or more of high mannose glycans, sialylated glycans, and 3,3,1,0,0 glycans relative to a corresponding level in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising DMSO.
A method of decreasing a level of one or more fucosylated glycans in a recombinant protein preparation, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising DMSO under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has a decreased level of one or more fucosylated glycans relative to a corresponding level in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising DMSO.
Dependencies are inferred from the claim text.