Noninvasive method for measuring oxidative stress and oxidative damage from skin: oxidative stress and oxidative damage biomarkers

A noninvasive method for diagnosing oxidative stress and oxidative damage in a subject comprising:

• a. Applying an adhesive article to an epithelium of a mammal;
• b. Allowing for adherence of epithelial cells to the adhesive article;
• c) removing the adhesive article from the epithelium of the mammal;
• d) preparing the adhesive article using standard laboratory methods for extraction;
• e) extracting a biomarker selected from the group consisting of Myeloperoxidase and oxidized lipids from the epithelial cells adhered to said adhesive article;
• f) measuring the biomarkers from the epithelial cells adhered to said adhesive article;
• g) determining the amount of the biomarker in the epithelial cells as compared to a baseline sample following a treatment.