Method of detecting Mycobacterium tuberculosis complex by cell filtrate protein 10-loaded detonation nanodiamond
AssigneeNational Dong Hwa University
InventorsWen-Ping Peng, Po-Chi Soo, Ching-Jen Kung, Kou-Hwa Chang, Shu-Chuan Yu, Wan-Zo Ho
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Abstract
A method of detecting Mycobacterium tuberculosis complex (MTBC) in a culture media is provided, in which, the culture media containing the MTBC and a biomarker, such as CFP-10, secreted from the MTBC is provided, the culture media is filtered to obtain a filtrate, detonation nanodiamond particles are mixed with the filtrate to form biomarker-loaded detonation nanodiamond particles, and a mass spectrometry analysis process is performed on the biomarker-loaded detonation nanodiamond particles for detecting the biomarker.
Claims19
A method of detecting Mycobacterium tuberculosis complex in a culture media, comprising:
- providing the culture media containing the Mycobacterium tuberculosis complex and a biomarker secreted from the Mycobacterium tuberculosis complex;
- filtering the culture media to obtain a filtrate;
- mixing detonation nanodiamond (DND) particles with the filtrate to form biomarker-loaded DND particles;
- and
- performing a first mass spectrometry analysis process on the biomarker-loaded DND particles for detecting the biomarker.
A method of detecting Mycobacterium tuberculosis complex (MTBC) in a clinical isolate, comprising:
- providing the clinical isolate;
- culturing the clinical isolate in a broth culture media for MTBC to secrete biomarker molecules;
- filtering the broth culture media to obtain a filtrate substantially free of cells;
- mixing detonation nanodiamond (DND) particles with the filtrate for allowing the DND particles to absorb the biomarker molecules;
- and
- performing a first mass spectrometry analysis process on the DND particles absorbing the biomarker molecules for detecting the biomarker molecules.
A cell filtrate protein 10 (CFP-10)-loaded detonation nanodiamond (DND) particle for detecting a clinical isolate of Mycobacterium tuberculosis complex in a culture media by mass spectrometry, comprising:
- a detonation nanodiamond particle;
- and
- at least a molecule of CFP-10 absorbed by the detonation nanodiamond particle.
Dependencies are inferred from the claim text.