Method for producing recombinant prothrombin, vector dna, and reagent kit
AssigneeSysmex Corporation
InventorsTakahiro BANDO, Mutsumi SUGAI
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Abstract
The present invention provides a method for producing recombinant prothrombin. The method comprises: providing a vector DNA into which a gene encoding a tag and a gene encoding prothrombin are incorporated, wherein the tag is selected from the group consisting of MBP, SUMO, and NusA; and expressing a tag fusion type prothrombin in a lepidopteran insect or cultured cells of the lepidopteran insect.
Claims20
A method for producing recombinant prothrombin, comprising:
- providing a vector DNA into which a gene encoding a tag and a gene encoding prothrombin are incorporated, wherein the tag is selected from the group consisting of MBP, SUMO, and NusA;
- and
- expressing a tag fusion type prothrombin in a lepidopteran insect or cultured cells of the lepidopteran insect.
A vector DNA into which a gene encoding a tag and a gene encoding prothrombin are incorporated, wherein the tag is selected from the group consisting of MBP, SUMO, and NusA.
A reagent kit comprising:
- a thrombin reagent comprising a thrombin;
- and
- a diluent buffer for diluting plasma of a subject,
- wherein the thrombin is obtained from a tag fusion type prothrombin which is expressed in a lepidopteran insect or cultured cells of the lepidopteran insect using a vector DNA into which a gene encoding a tag and a gene encoding prothrombin are incorporated, wherein the tag is selected from the group consisting of MBP, SUMO, and NusA.
Dependencies are inferred from the claim text.