Assay for clostridium botulinum neurotoxin
AssigneeAlbert-Ludwigs-Universität Freiburg
InventorsGregory Stevens, Michael Krueger, Andrea Zgaga-Griesz, Gerald Urban, Dalice Silver
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Abstract
The present invention relates to a method of determining Botulinum toxin (BoNT) based on a luminescence assay. The present application further relates to a peptide that is susceptible to proteolytic cleavage by BoNT which is suitable for that method.
Claims23
A method of determining the presence or amount of Botulinum toxin (BoNT) in a test sample, said method comprising the steps of
- a) providing a support and a peptide for attachment thereto, said peptide comprising:
- an amino acid sequence susceptible to proteolytic cleavage by BoNT,
- an amino acid sequence corresponding to a luciferase, and
- a tag suitable for attaching the peptide to said support,
- b) attaching said peptide to said support,
- c) adding the test sample to be investigated for presence or amount of BoNT, and
- d) determining the luminescence signal of the luciferase.
A peptide comprising an amino acid sequence susceptible to proteolytic cleavage by BoNT, wherein said peptide comprises a Halo-tag, two sets of amino acids amino acids 146-206, of SNAP25, and luciferase in series.
Dependencies are inferred from the claim text.