In-vitro method for monoclonal antibody production

Abstract

A method of producing an IgG type monoclonal antibody in vitro is described. The method includes the steps of: (A) providing B-cells obtained from a transgenic non-human mammal whose genome comprises a disruption of an Act1 gene; (B) contacting the B cells with an antigen under conditions which result in formation of an antibody specific to the antigen by the B cells; (C) fusing the B cells with one or more immortal cells, to produce one or more hybridomas which express IgG type monoclonal antibodies that specifically bind to the antigen; (D) selecting a hybridoma which expresses an antibody specific to the antigen; and (E) obtaining monoclonal antibody produced by the selected hybridoma. Kits for carrying out the method of producing an IgG type monoclonal antibody are also described.

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