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US 2014/0273029 A1Published application
Methods and compositions for enhancing immunoassays
AssigneeThe Board of Trustees of the University of Illinois
InventorsRyan C. Bailey, Matthew S. Luchansky, Abraham J. Qavi, Jared T. Kindt
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Abstract
Embodiments of the methods, compositions, and systems provided herein relate to enzymatic enhancement of immunoassays using photonic sensor arrays.
Claims6
A method of detecting a target analyte comprising:
- (a) obtaining an optical ring resonator having a first anti-cytokine antibody attached thereto, wherein the first anti-cytokine antibody is anti-IL-2 from clone 555051 antibody;
- (b) contacting the first anti-cytokine antibody with a sample comprising IL-2 that selectively binds to the anti-cytokine antibody;
- (c) contacting the bound IL-2 with a second anti-cytokine antibody that selectively binds to the cytokine, wherein the second anti-cytokine antibody is a biotinylated anti-IL-2 from clone 555040 antibody;
- (d) contacting the biolinylated second anti-cytokine antibody with a streptavidin-horseradish peroxidase conjugate;
- (e) contacting the horseradish peroxidase with 4-chloro-1-naphthol and hydrogen peroxide under conditions that oxidize 4-chloro-1-naphthol to 4-chloro-1-naphthon, whereby the 4-chloro-1-naphthon precipitates on the surface of the optical ring resonator;
- and
- (f) measuring a change in resonance wavelengths of the optical ring resonator, thereby indicating the presence of the cytokine.
4 more elements
A method of detecting a target analyte comprising:
- (a) obtaining an optical ring resonator having a first anti-cytokine antibody attached thereto, wherein the first anti-cytokine antibody is anti-IL-6 from clone MAB206 antibody;
- (b) contacting the first anti-cytokine antibody with a sample comprising IL-6 that selectively binds to the anti-cytokine antibody;
- (c) contacting the bound IL-2 with a second anti-cytokine antibody that selectively binds to the cytokine, wherein the second anti-cytokine antibody is a biotinylated anti-IL-6 from clone BAF206;
- (d) contacting the biolinylated second anti-cytokine antibody with a streptavidin-horseradish peroxidase conjugate;
- (e) contacting the horseradish peroxidase with 4-chloro-1-naphthol and hydrogen peroxide under conditions that oxidize 4-chloro-1-naphthol to 4-chloro-1-naphthon, whereby the 4-chloro-1-naphthon precipitates on the surface of the optical ring resonator;
- and
- (f) measuring a change in resonance wavelengths of the optical ring resonator, thereby indicating the presence of the cytokine.
4 more elements
Dependencies are inferred from the claim text.