Biomarkers for detecting the presence of bacteria
AssigneeSilver Lake Research Corporation
InventorsRobert K. DiNello, Mark Geisberg
Life of this patent
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Abstract
In some aspects, provided are methods relating to the use of bacterial N-methyl-2 superfamily proteins as a biomarker for the presence of bacteria in a sample. The invention also relates to novel methods of diagnosis of the presence of bacteria in a liquid or solid sample, detection of bacterial infections in humans or animals, and use of antibodies or other specific binding molecules capable of binding to N-methyl-2 superfamily proteins.
Claims44
A method of detecting the presence of bacteria in a sample comprising:
- (a) contacting the sample with an antibody, fragment thereof, aptamer or ligand capable of binding a protein in the N-methyl-2 superfamily to form one or more complexes in the presence of the N-methyl-2 superfamily proteins, if any, in the sample;
- and
- (b) detecting the presence of the one or more said complexes,
- wherein the presence of at least one complex indicates the presence of bacteria.
A method of detecting bacterial contamination in a sample comprising:
- (a) contacting the sample with an antibody, fragment thereof, aptamer or ligand capable of binding a protein in the N-methyl-2 superfamily to form one or more complexes in the presence of the N-methyl-2 superfamily proteins, if any, in the sample;
- and
- (b) detecting the one or more said complexes,
- wherein the presence of at least one complex indicates the presence of a contaminating concentration of bacteria.
A method of identifying the presence of one or more specific bacteria in a sample comprising:
- (a) contacting the sample with an antibody, fragment thereof, aptamer or ligand capable of binding one or more identified proteins of the N-methyl-2 superfamily expressed by a subset of bacteria but not by other bacteria to form one or more complexes in the presence of the one or more specific N-methyl-2 superfamily proteins, if any, in the sample;
- and
- (b) detecting the presence of the one or more said complexes,
- wherein the presence of at least one complex indicates the presence of the one or more specific bacteria.
The method of 26, wherein the solid support is a particle, a bead, a plastic or glass surface, a porous membrane, an array, or a chip.
Dependencies are inferred from the claim text.