Magnecytometer For The Detection Of One Or More Analytes
AssigneeThe Regents of the University of New Mexico
InventorLaurel Sillerud
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Abstract
A system and method for analyzing a sample of liquid having an NMR signal in response to a magnetic field for the presence of an analyte. Included is an NMR device having a testing section that is adapted to contain a liquid and apply a magnetic field to the liquid. A complex comprised of a conjugate having a field gradient bound to the analyte that is of sufficient magnitude to quench the NMR signal of the liquid when in the test section whereby the presence of the complex is determined by the absence of the NMR signal.
Claims20
A method for analyzing a sample of liquid having an NMR signal in response to a magnetic field for the presence of an analyte, the method comprising the steps of:
- conjugating said analyte with a conjugate, said conjugate having a field gradient that quenches the NMR signal of the liquid;
- placing said analyte in a sample testing section of a nuclear magnetic resonance device;
- applying a magnetic field to said analyte when said analyte is located in said sample testing section;
- and
- determining the presence of said analyte based upon said NMR signal of the liquid being quenched by said conjugate.
A method for using a nuclear magnetic resonance device to count one or more analytes in a sample liquid having an NMR signal in response to a magnetic field comprising the steps of:
- attaching a biological marker to said analytes;
- testing for said biological marker prior to attaching said analytes to an affinity column;
- testing for said biological marker after said one or more analytes pass through said affinity column;
- continuing to pass said one or more analytes through said column until no analytes are detected passing through the column to create an elution solution;
- said elution solution containing one or more biological complexes comprised of analytes bound to recognition ligands, said biological complexes formed by conjugating a conjugate to said one or more analytes, said conjugate having a field gradient that quenches the NMR signal of the liquid;
- attaching said one or more biological complexes to said affinity column;
- passing said elution solution through a nuclear magnetic resonance device having a sample testing section in which a magnetic field is applied;
- applying a magnetic field to said one or more biological complexes when said biological complexes are located in said sample testing section;
- and
- counting said biological complexes by determining the presence of a biological complex based upon said NMR signal from the liquid being quenched by said conjugate.
A system for analyzing a sample of liquid having an NMR signal in response to a magnetic field for the presence of an analyte, comprising:
- an NMR device having a testing section adapted to contain said liquid and apply a magnetic field to said liquid when said liquid is in said testing section;
- a complex comprised of a conjugate having a field gradient bound to said analyte;
- and
- said field gradient of sufficient magnitude to quench the NMR signal of said liquid when said liquid in said testing section whereby the presence of the complex is determined from the absence of said NMR signal of said liquid.
Dependencies are inferred from the claim text.