Sample Preparation Device and Methods of Use
AssigneeF Cubed, LLC
InventorsLeslie T. Ivie, Christopher Chanelli, Shaunasee Kocen
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Abstract
A device for isolating DNA from a sample containing cells, including a cartridge having an entrance port and an exit port, a membrane disposed between the entrance port and the exit port, and a plurality of channels between the membrane and the exit port. Additionally, systems and methods for isolating DNA from a sample containing cells and also systems and methods for amplifying and isolating single-stranded DNA from a sample containing DNA.
Claims52
A device for isolating DNA from a sample containing cells, comprising
- a cartridge having an entrance port and an exit port;
- a membrane disposed between the entrance port and the exit port;
- and
- a plurality of channels between the membrane and the exit port.
A method of processing DNA from a sample, comprising
- providing a sample containing cells having DNA;
- transferring the sample into a cartridge having a membrane and a plurality of channels disposed therein;
- lysing the cells to release the DNA and non-DNA cellular material;
- passing the DNA through the membrane;
- and
- passing the DNA through the channels;
- wherein the DNA is isolated from the sample.
A method of amplifying and isolating single-stranded DNA from a sample containing DNA, comprising
- providing a sample containing DNA;
- amplifying the DNA via a polymerase chain reaction (PCR), wherein the PCR is asymmetric PCR, to produce a mixture of double-stranded DNA and single-stranded DNA;
- providing a magnetic substrate having an affinity for binding the double-stranded DNA;
- binding the magnetic substrate to the double-stranded DNA;
- applying a magnetic field to the magnetic substrate, wherein the magnetic field attracts the magnetic substrate bound to the double-stranded DNA;
- and
- isolating the single-stranded DNA from the mixture.
Dependencies are inferred from the claim text.