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US 2014/0272984 A1Published application
Microfluidic Flow Monitoring
AssigneeCanon U.S. Life Sciences, Inc.
InventorsKenton C. Hasson, Gregory A. Dale
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Abstract
The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system.
Claims51
A system for monitoring a solution flow in a microfluidic channel comprising:
- (a) a chip comprising at least one microfluidic channel;
- (b) a fluid movement system for moving a fluid through the channel to fill the at least one channel with a solution and stop the solution flow;
- (c) a flow marker introduction system for introducing a flow marker into the channel;
- (d) an illumination system for generating at least two measuring points;
- and
- (e) a detection system for measuring the movement of the flow marker within the channel between the at least two measuring points.
3 more elements
(canceled)
A method of monitoring a solution flow in a microfluidic channel comprising:
- (a) moving a solution through the microfluidic channel in a chip to fill the channel with a solution and stop the solution flow;
- (b) introducing a flow marker into the channel;
- (c) illuminating the channel at two measuring points;
- and
- (d) measuring the movement of the flow marker within the channel between the two measuring points.
2 more elements
(canceled)
Dependencies are inferred from the claim text.