Unheated extraction of genomic dna in an automated laboratory system
AssigneeHealth Diagnostic Laboratory, Inc.
InventorRoss HIGGINS
Life of this patent
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Abstract
A method for analyzing genomic DNA includes introducing a plurality of samples comprising human cells into individual vessels in each of a plurality of multi-vessel well plates. At least a subset of the human cells in the plurality of samples is lysed without the use of heat. DNA in the at least a subset of lysed human cells is isolated with the use of a plurality of paramagnetic beads. The isolated DNA is analyzed to identify one or more single nucleotide polymorphisms (SNPs), wherein the lysing, isolating, and analyzing steps are performed substantially in parallel for each of the plurality of samples.
Claims18
A method for analyzing genomic DNA, the method comprising:
- introducing a plurality of samples comprising human cells into individual vessels in each of a plurality of multi-vessel well plates;
- lysing at least a subset of the human cells in the plurality of samples without the use of heat;
- isolating DNA in the at least a subset of lysed human cells with the use of a plurality of paramagnetic beads;
- and
- analyzing the isolated DNA to identify one or more single nucleotide polymorphisms (SNPs), wherein the lysing, isolating, and analyzing steps are performed substantially in parallel for each of the plurality of samples.
Dependencies are inferred from the claim text.