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US 2014/0272967 A1Published application
Systems and methods for isolating nucleic acids
AssigneeAbbott Molecular Inc.
InventorsGerard Gundling, Robert Kowal, Edward Granados, Natalie Solomon, Wai-Bing Mak, Magdalena Szostak
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Abstract
The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating low molecular weight circulating nucleic acids from bodily fluids (e.g., plasma).
Claims20
A method of isolating nucleic acids, comprising:
- a) contacting a sample comprising said nucleic acids with lysis buffer comprising greater than 35% ethanol by volume to lyse said cells to generate lysed cells;
- and
- b) isolating said nucleic acid from said lysed cells.
A kit, comprising:
- a) a lysis buffer comprising 35% or more ethanol by volume;
- and
- b) a solid support.
A composition, comprising:
- a circulating DNA;
- and a lysis buffer comprising 35% or more ethanol by volume.
Dependencies are inferred from the claim text.