Blue Fluorescent Protein and Methods of Use Thereof
AssigneeWiSys Technology Foundation, Inc.
InventorsWayne Schaefer, Ramaswamy Subramanian, Daniel Ferraro, Chi Li Yu, David Gibson, Swagatha Ghosh, P. Sai Sudha
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Abstract
This invention provides methods of making and using a fluorescent probe from the Sandercyanin protein as set forth in SEQ ID NO: 1 or SEQ ID NO: 2. In one embodiment, the invention provides a method of creating a fluorescent probe, comprising the steps of attaching a Sandercyanin moiety to a probe, wherein the probe is specific to a desired target.
Claims14
A method of preparing a fluorescent probe, the method comprising attaching a Sandercyanin moiety or a fragment thereof to a probe for detecting a specific target.
A fluorescent probe, comprising a Sandercyanin moiety or a fragment thereof and a probe for detecting a specific target.
A labeled marker for detection of a target, the marker comprising a label selected from the group consisting of Sandercyanin moiety or a fragment thereof and a fluorescent variant thereof, and a ligand configured to bind to the target.
A method for detecting a target comprising:
- a. providing a labeled ligand which incorporates a label selected from the group consisting of Sandercyanin fluorescent protein or a fragment thereof and a fluorescent variant thereof, and a ligand for binding the target;
- b. contacting the target with the labeled ligand;
- c. allowing the labeled ligand to bind to the target;
- d. subjecting the labeled ligand and target to light having a wavelength which excites the label;
- and
- e. observing the locus of fluorescence.
A method for producing an isolated recombinant protein comprising introducing DNA encoding Sandercyanin protein or a fragment thereof into the organism, culturing the organism in an enclosed system, harvesting the organism, and isolating the recombinant protein from the organism.
Dependencies are inferred from the claim text.