Methods of Hybridizing Probes to Genomic DNA

A method of improving binding efficiency of a labeled probe to double stranded DNA having a portion of the double stranded DNA separated into a first single strand segment and a complementary single strand segment comprising

• combining the double stranded DNA with a labeled probe that is complementary to the first single strand segment at a target sequence and one or more anti-lock probes that are complementary to either the first single strand segment or the complementary single strand segment wherein the labeled probe binds to the first single strand segment at the target sequence and the one or more anti-lock probes bind to at least the complementary single strand segment.