Methods and Systems For DNA-Based Detection And Reporting
AssigneeSRC, Inc.
InventorsAdam J. Lowe, Jesse W. Taylor
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Abstract
Methods, systems, and devices for the detection and notification of chemical, biological, radiological, nuclear, and/or explosive agent. The detection and notification system uses aptamers bound to a catalyst that is activated when the aptamer binds to the agent. The activated catalyst acts on a substrate to create a signal that is detected, indicating the presence of the agent. For example, the system can be a glove worn by a user that includes a catalyst/substrate pair that, in the presence of the target agent, produces a colorimetric and/or fluorescent signal in a time-efficient manner.
Claims30
A method of detecting a target in a sample using an article held or worn by a user, the article comprising a target sensor, wherein the target sensor comprises a plurality of aptamers capable of binding to the target and comprising a catalyst capable of an inactive, aptamer-bound state and an active state, and further wherein the target sensor comprises a substrate configured to be acted on by the catalyst only in said active state, the method comprising the steps of:
- contacting the article with the sample, wherein the catalyst switches from the inactive, aptamer-bound state to the active state in response to binding of the target to the plurality of aptamers, and further wherein the active state catalyst interacts with the substrate to produce a notification signal;
- detecting said notification signal, wherein said signal indicates the presence of said target in said sample.
A method of detecting a chemical, biological, radiological, or explosive target in a sample using an article of clothing worn by a user, the article comprising a target sensor, wherein the target sensor comprises a plurality of aptamers capable of binding to the target and comprising a catalyst capable of an inactive, aptamer-bound state and an active state, and further wherein the target sensor comprises a substrate configured to be acted on by the catalyst only in said active state, the method comprising the steps of:
- contacting the article with the sample, wherein the catalyst switches from the inactive, aptamer-bound state to the active state in response to binding of the target to the plurality of aptamers, and further wherein the active state catalyst interacts with the substrate to produce a colorimetric or fluorescent notification signal;
- detecting said colorimetric or fluorescent notification signal, wherein said signal indicates the presence of said target in said sample.
A system for detecting a target in a sample using an article held or worn by a user, the system comprising:
- a sample, said sample potentially comprising said target;
- and
- an article, the article comprising a sensor and a substrate, wherein the sensor comprises a plurality of aptamers, said plurality of aptamers capable of binding to the target and comprising a catalyst capable of an inactive, aptamer-bound state and an active state, and wherein the substrate is configured to be acted on by the catalyst only when said catalyst is in said active state;
- wherein said sensor is configured to generate a notification signal when said active state catalyst interacts with the substrate.
A system for detecting a chemical, biological, radiological, or explosive target in a sample using an article of clothing, the system comprising:
- a sample, said sample potentially comprising said target;
- and
- an article, the article comprising a sensor and a substrate, wherein the sensor comprises a plurality of aptamers, said plurality of aptamers capable of binding to the target and comprising a catalyst capable of an inactive, aptamer-bound state and an active state, and wherein the substrate is configured to be acted on by the catalyst only when said catalyst is in said active state;
- wherein said sensor is configured to generate a colorimetric or fluorescent notification signal when said active state catalyst interacts with the substrate.
Dependencies are inferred from the claim text.