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US 2014/0272944 A1Published application
One-step procedure for the purification of nucleic acids
AssigneeAbbott Molecular Inc.
InventorGerard J. Gundling
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Abstract
The present invention is a new and non-obvious method for the improved and simplified purification of nucleic acids.
Claims24
A method of removing lysis buffer components from a nucleic acid sample, the method comprising
- a) providing a specimen comprising or suspected of comprising nucleic acids,
- b) contacting the specimen with one or more lysis reagents,
- c) contacting the specimen with a solid substrate for a length of time and under conditions suitable for nucleic acids in the specimen to bind to the solid substrate and
- d) causing the solid substrate bound with nucleic acids, if nucleic acid is present in the specimen, to pass through an aqueous-based separation gel.
1 more element
A method for enriching nucleic acids from a sample, the method comprising:
- a) providing;
- i) a sample suspected of comprising nucleic acids,
- ii) microparticles suitable for binding nucleic acids, and
- iii) an aqueous-based separation gel,
- b) contacting the sample with the microparticles for a length of time and under conditions suitable for any nucleic acids in the sample to bind to the magnetic microparticles to create loaded magnetic microparticles;
- c) drawing the loaded microparticles through the aqueous-based separation gel with a magnetic field.
3 more elements
Dependencies are inferred from the claim text.