Back to results
US 2014/0272943 A1Published application
Rapid and highly fieldable viral diagnostic
AssigneeUT-Battelle, LLC
InventorTimothy E. McKnight
Life of this patent
Estimating the term…
Abstract
The present invention relates to a rapid, highly fieldable, nearly reagentless diagnostic to identify active RNA viral replication in a live, infected cells, and more particularly in leukocytes and tissue samples (including biopsies and nasal swabs) using an array of a plurality of vertically-aligned nanostructures that impale the cells and introduce a DNA reporter construct that is expressed and amplified in the presence of active viral replication.
Claims39
A method for rapid diagnosis of RNA viral infection in a sample which comprises
- (a) adsorbing either
- (i) a sample of blood onto a porous filter to entrap leukocytes but permit gravimetric flow through of platelets and red blood cells, thereby obtaining a sample of leukocytes,
- (ii) a tissue sample onto a filter or other surface,
- (iii) a biological fluid sample onto a filter or other surface, or
- (iv) a sample of leukocytes onto a filter or other surface;
- (b) impaling cells in said sample on the filter or surface onto an array of a plurality of vertically aligned nanostructures carrying at least one DNA reporter construct thereon, wherein the at least one DNA reporter encodes an mRNA viral replicon, which comprises cis-acting RNA viral replication elements and any other adjacent regions necessary for viral replication flanking and operably linked to a reporter gene or operon, and which elements are capable of complementation in trans with viral replication factors in virally-infected cells to thereby amplify the replicon;
- and
- (c) detecting expression of said reporter gene or operon when virally-infected cells are present.
5 more elements
A method for rapid diagnosis of RNA viral infection which comprises
- (a) impaling tissue or a tissue lesion obtained from a patient, said tissue or tissue lesion being suspected of RNA viral infection, onto an array of a plurality of vertically aligned nanostructures carrying at least one DNA reporter construct thereon, wherein the at least one DNA reporter encodes an mRNA viral replicon, which comprises cis-acting RNA viral replication elements and any other adjacent regions necessary for viral replication flanking and operably linked to a reporter gene or operon, and which elements are capable of complementation in trans with viral replication factors in virally-infected cells to thereby amplify the replicon;
- and
- (b) detecting expression of said reporter gene or operon when virally-infected cells are present.
Dependencies are inferred from the claim text.