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US 2014/0272939 A1Published application
Assay methods
AssigneeMeso Scale Technologies, LLC.
InventorsAnahit Aghvanyan, Eli N. Glezer, John Kenten, George Sigal, Martin Stengelin
Life of this patent
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Abstract
The present invention is directed to methods for improving assay specificity and performance in binding assays.
Claims60
A method of detecting an analyte of interest in a sample comprising:
- a. binding the analyte to:
- (i) a capture reagent on a surface comprising the capture reagent for the analyte, and an anchoring reagent comprising an anchoring oligonucleotide sequence;
- (ii) a first detection reagent for the analyte that is linked to a first nucleic acid probe;
- and (iii) a second detection reagent for the analyte that is linked to a second nucleic acid probe;
- thereby forming a complex on the surface comprising the binding reagent, the analyte and the first and second detection reagents;
- b. using an extension process that requires the first and second probes to be in proximity, extending the second probe to form an extended sequence comprising an anchoring sequence complement that is complementary to the anchoring sequence;
- c. hybridizing the anchoring sequence to the anchoring sequence complement;
- and
- d. measuring the amount of extended sequence bound to the surface.
6 more elements
A kit for the detection of an analyte of interest in a sample comprising, in one or more vials, containers, or compartments:
- a. a surface comprising (i) a capture reagent for the analyte, and (ii) an anchoring reagent comprising an anchoring oligonucleotide sequence;
- b. a first detection reagent for the analyte that is linked to a first nucleic acid probe;
- and
- c. a second detection reagent for the analyte that is linked to a second nucleic acid probe.
1 more element
Dependencies are inferred from the claim text.