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US 2014/0272937 A1Published application
Low Resource Processor Using Surface Tension Valves for Extracting, Concentrating, and Detecting Whole Cells
AssigneeVanderbilt University
InventorsRick Haselton, David Wright, Joseph Conrad, Nick Adams
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Abstract
Systems and methods are described for isolation, separation and detection of a molecular species using a low resource device for processing of samples. Methods include isolation, separation and detection of whole cells.
Claims52
A method of processing a cell-containing sample comprising:
- (a) providing a device comprising a plurality of sequential chambers connected by tubing, each of said sequential chambers comprising a fluid and separated by gas-based surface tension valves, wherein a first reaction chamber comprises a particle having a reactant on its surface;
- (b) introducing into said first reaction chamber a sample comprising at least one whole cell;
- (c) incubating said first reaction chamber under conditions sufficient to permit reaction of said reactant with said at least one whole cell in said sample;
- (d) transporting said particle from said first reaction chamber into at least a second chamber through tubing disposed therebetween;
- and
- (e) detecting interaction of said at least one whole cell with said reactant.
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A method of processing a cell-containing sample comprising:
- (a) providing a device comprising a plurality of sequential chambers connected by tubing, each of said sequential chambers comprising a fluid and separated by gas-based surface tension valves;
- (b) introducing into said first chamber a particle comprising a surface reactant, the surface of which comprises at least one whole cell bound to said reactant;
- (c) transporting said particle from said first chamber into at least a second chamber through tubing disposed therebetween;
- and
- (d) detecting the presence of said at least one whole cell.
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Dependencies are inferred from the claim text.