US 2014/0271944 A1Published application

Compositions for Alleviating Inflammation and Oxidative Stress in a Mammal

AssigneeLifeline Nutraceuticals Corporation

InventorsJoe Milton McCord, William J. Driscoll

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Abstract

An antioxidant-promoting composition that increases antioxidant defense potential in a subject is disclosed. The composition contains Bacopa monniera extract comprising a Bacopa monniera active ingredient; a Silybum marianum (milk thistle) extract comprising a Silybum marianum active ingredient; a Withania somnifera (ashwagandha) extract comprising a Withania somnifera active ingredient; a Camellia sinensis (green tea) extract comprising a Camellia sinensis active ingredient; a Curcuma longa (turmeric) extract comprising at least one Curcuma longa active ingredient, and optionally a Centella asiatica (Gotu kola) extract; a Ginko biloba extract; an Aloe vera extract; and N-acetyl cysteine. The process for quantifying Nrf2 transcription factor activating potential of the botanical extracts comprising the botanical active ingredients is described.

Claims23

Dependent claims

A composition for treating oxidative stress in a subject in need thereof, the composition comprising at least two botanical extracts, each botanical extract containing a therapeutic amount of a botanical active ingredient, wherein the botanical extract is selected from the group consisting of:

(a) a Bacopa monniera extract comprising at least one Bacopa monniera active ingredient;
(b) a Silybum marianum (milk thistle) extract comprising at least one Silybum marianum active ingredient;
(c) a Withania somnifera (ashwagandha) extract comprising at least one Withania somnifera active ingredient;
(d) a Camellia sinensis (green tea) extract comprising at least one Camellia sinensis active ingredient;
and
(e) a Curcuma longa (turmeric) extract comprising at least one Curcuma longa active ingredient;
wherein the therapeutic amount of the botanical active ingredient is effective to:
(i) increase a level of enzyme activity of at least one antioxidant enzyme selected from the group consisting of a superoxide dismutase (SOD), a catalase (CAT), a glutathione peroxidase (GPX);
(ii) decrease a level of reactive oxygen species (ROS);
(iii) synergistically increase expression of a phase 2 gene by activating a Nuclear factor-erythroid-2-related factor 2 (Nrf2) transcription factor-dependent transcription activity of an antioxidant response element (ARE);
or a combination thereof,
without significant side effects thereby treating the oxidative stress.

9 more elements

A method of quantifying a Nuclear factor-erythroid-2-related factor 2 (Nrf2) transcription factor activating potential of a botanical extract comprising a botanical active ingredient, comprising:

(1) contacting a first sample of AREc32 cells with the botanical extract and a second sample of AREc32 cells with a buffer control;
(2) incubating the first sample of AREc32 cells with the botanical extract and the second sample of AREc32 cells with the buffer control for at least 18 hours at 37° C.;
(3) washing the first and second samples of AREc32 cells from step (2);
(4) lysing the first and second samples of washed AREc32 cells from step (3);
(5) incubating the lysate from the first and second samples of lysed AREc32 cells from step (4) with D-luciferin;
and
(6) measuring luminescence of the first and second samples of incubated AREc32 cells from step (5).

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 13/829,205Filed 14 Mar 2013
Published: 18 Sep 2014
Term ends: Not estimatedThe source records don’t support an estimate.

Classification22 codes

A61K 36/28
Magnoliopsida: asteraceae or Compositae , e.g. chamomile, feverfew, yarrow or echinacea
A61K 31/12
Medicinal preparations containing organic active ingredients: ketones
A61K 31/198
The amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine,…:…
A61K 31/353
Condensed with carbocyclic rings, e.g. methantheline: 3,4-Dihydrobenzopyrans, e.g. chroman, catechin