Particulate hyaluronic acid formulations for cellular delivery of bioactive agents
AssigneeAgency for Science, Technology and Research
InventorsMotoichi KURISAWA, Shengyong NG, Joo Eun CHUNG
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Abstract
There is presently provided a suspension of immiscible particles in a solution, wherein the particles comprise an agglomeration of a bioactive agent; and a plurality of conjugates of a hyaluronic acid and a flavonoid wherein the particles are on average from about 15 nm to about 300 nm in diameter and wherein the bioactive agent is releasably retained in the particles by the flavonoid. There is also provided a therapeutic formulation comprising such a suspension and methods for using the suspension and therapeutic formulation, including for delivery of a bioactive agent to a cell and for treating a disease, including cancer.
Claims14
1-17. (canceled)
A method for delivery of a bioactive agent to a cell, the method comprising contacting the cell with a suspension of immiscible particles in a solution, wherein the particles comprise an agglomeration of:
- a bioactive agent;
- and
- a plurality of conjugates of a hyaluronic acid and a flavonoid;
- wherein the particles are on average from about 15 nm to about 300 nm in diameter and wherein the bioactive agent is releasably retained in the particles by the flavonoid.
(canceled)
24-31. (canceled)
A method of formulating a suspension of immiscible particles comprising combining in a solution:
- a. 0.01% w/v to about 1.0% w/v of a bioactive agent;
- and
- b. 0.05 μg/ml to about 5000 μg/ml of conjugates of a hyaluronic acid and a flavonoid;
- to form the suspension of particles wherein the particles comprise an agglomeration of the bioactive agent and the conjugates of hyaluronic acid and a flavonoid, wherein the particles are on average from about 15 nm to about 300 nm in diameter and wherein the bioactive agent is releasably retained in the particles by the flavonoid.
33-44. (canceled)
(canceled)
48-49. (canceled)
Dependencies are inferred from the claim text.