Porcine reproductive and respiratory syndrome virus, compositions, vaccine and methods of use
AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsDianna M. Murphy JORDAN, Brian Thomas MARTINSON, Michael B. ROOF, Eric Martin VAUGHN, Joseph Gilbert VICTORIA
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Abstract
The present invention relates to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) mutant strains having increased stability; compositions containing the strains or antigens derived therefrom; vaccines containing the strains, including killed, attenuated or subunit vaccines; nucleic acids encoding PRRSV polypeptides, polypeptides encoded by the nucleic acids, including antigenic fragments thereof; antibodies which specifically bind to said polypeptides, and methods making and using thereof.
Claims20
An immunogenic composition of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), comprising at least one of
- a) a composition comprising a polynucleotide which is
- i) a polynucleotide of SEQ ID NO:1 (preattenuated Wetzel p3) or
- ii) a polynucleotide of SEQ ID NO:2 (attenuated Wetzel p41);
- b) an immunogenic fragment of a polynucleotide of SEQ ID NO:1 or SEQ ID NO:2;
- c) a polypeptide selected from the group consisting of
- i) SEQ ID NO:3 (ORF1a protein of Wetzel p3)
- ii) SEQ ID NO:4 (ORF1b protein of Wetzel p3),
- iii) SEQ ID NO:5 (GP2 protein of Wetzel p3),
- iv) SEQ ID NO:6 (GP3 protein of Wetzel p3),
- v) SEQ ID NO:7 (GP4 protein of Wetzel p3 and of Wetzel p41),
- vi) SEQ ID NO:8 (GP5 protein of Wetzel p3),
- vii) SEQ ID NO:9 (M protein of Wetzel p3 and of Wetzel p41),
- viii) SEQ ID NO:10 (N protein of Wetzel p3 and of Wetzel p41),
- ix) SEQ ID NO:11 (ORF1a protein of Wetzel p41),
- x) SEQ ID NO:12 (ORF1b protein of Wetzel p41),
- xi) SEQ ID NO:13 (GP2 protein of Wetzel p41),
- xii) SEQ ID NO:14 (GP3 protein of Wetzel p41), and
- xiii) SEQ ID NO:15 (GP5 protein of Wetzel p41);
- or
- d) an immunogenic fragment of a polypeptide of SEQ ID NOs:
- 3-15.
An isolated polynucleotide, comprising a polynucleotide
- a) having the nucleic acid sequence of SEQ ID NO:1 (preattenuated Wetzel p3) or SEQ ID NO:2 (attenuated Wetzel p41);
- b) having a nucleic acid sequence that encodes a polypeptide of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15;
- c) having a nucleic acid sequence 95% identical to SEQ ID NO:1 or SEQ ID NO:2, and which encodes a polypeptide having a biological or immunologically-effective activity of a polypeptide of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15;
- d) that is a fragment of the nucleic acid sequence of SEQ ID NO:1 or SEQ ID NO:2, comprising at least 30 contiguous nucleotide sequences of SEQ ID NO:1, or
- e) that is a fragment of the nucleic acid sequence of SEQ ID NO:1 or SEQ ID NO:2, comprising at least 30 contiguous nucleotide sequences of SEQ ID NO:1 or SEQ ID NO:2, and which encode an immunologically-effective activity of an amino acid sequence of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15.
An isolated polypeptide, comprising a polypeptide
- a) having the amino acid sequence of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15;
- b) having an amino acid sequence 95% identical to SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15 and having a biological or immunologically-effective activity of a polypeptide encoded by SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15, respectively;
- c) that is a fragment of the amino acid sequence of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15, comprising at least 15 contiguous amino acids of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15, respectively;
- d) that is a fragment of the amino acid sequence of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15, comprising at least 15 contiguous amino acids of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15, respectively, and having an immunologically-effective activity;
- or
- e) that is a protein fragment that is encoded by a polynucleotide that comprises at least 15 consecutive nucleotides included in the sequences of SEQ ID NO:1 or SEQ ID NO:2.
An isolated Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), comprising
- a) a nucleic acid sequence of SEQ ID NO:1 or SEQ ID NO:2, or
- b) a nucleic acid sequence 95% identical to SEQ ID NO:1 or SEQ ID NO:2, which encodes a polypeptide having a biological or immunologically-effective activity of a polypeptide of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15.
An isolated antibody that specifically binds to a PRRSV polypeptide encoded by a polynucleotide having a sequence of SEQ ID NO:1 or SEQ ID NO:2, said polypeptide having an amino acid sequence of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15, and wherein said antibody does not bind to a polypeptide encoded by a different strain of PRRSV.
Dependencies are inferred from the claim text.