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US 2014/0271622 A1Published application
Methods of cell culture
AssigneeMomenta Pharmaceuticals, Inc.
InventorHolly Prentice
Life of this patent
Estimating the term…
Abstract
Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using copper, are described.
Claims33
A method of producing a recombinant protein preparation having a target value of one or more of galactosylated glycans and G0F glycans, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising copper under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has the target value of the one or more of galactosylated glycans and G0F glycans.
1 more element
A method of producing a recombinant protein preparation, the method comprising:
- (a) providing a target value of one or more of galactosylated glycans and G0F glycans;
- (b) providing a cell genetically engineered to express a recombinant protein;
- (c) culturing the cell in a culture medium comprising copper under conditions in which the cell expresses the recombinant protein;
- (d) harvesting a preparation of the recombinant protein produced by the cell;
- and
- (e) formulating the preparation into a drug product if the preparation meets the target value of the one or more of galactosylated glycans and G0F glycans.
3 more elements
A method of decreasing a level of galactosylated glycans in a recombinant protein preparation, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising an elevated level of copper under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has a decreased level of galactosylated glycans relative to a corresponding level in a preparation of the recombinant protein produced using the medium not comprising the elevated level of copper.
1 more element
A method of increasing a level of G0F glycans in a recombinant protein preparation, the method comprising:
- (a) providing a cell genetically engineered to express a recombinant protein;
- (b) culturing the cell in a culture medium comprising an elevated level of copper under conditions in which the cell expresses the recombinant protein;
- and
- (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has an increased level of G0F glycans relative to a corresponding level in a preparation of the recombinant protein produced using the medium not comprising the elevated level of copper.
1 more element
Dependencies are inferred from the claim text.