Compositions And Methods For Mesenchymal And/Or Chondrogenic Differentiation Of Stem Cells
OwnerHossein Nejadnik, Olga Lenkov +1inventors
InventorsHossein Nejadnik, Olga Lenkov, Heike Daldrup-Link
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Abstract
Chondrocytes and compositions including chondrocytes produced via methods that do not require the formation of embryoid bodies are disclosed herein. The cells and compositions disclosed herein are suitable for use in treating osteoarthritis and other cartilage disorders or injury, as well as for tissue regeneration, particularly cartilage regeneration.
Claims25
A method for differentiating one or more toti-, pluri-, or multi-potent stem cells into a mesenchymal lineage, the method comprising:
- a) culturing the toti-, pluri-, or multi-potent stem cells in a stem cell medium as adherent cells;
- and
- b) pre-differentiating the toti-, pluri-, or multi-potent stem cells into toti-, pluri-, or multi-potent stem cell-derived mesenchymal stem cell (MSC)-like cells in a MSC medium, wherein the method does not result in the formation of embryoid bodies.
A composition for the treating a cartilage disease or disorder comprising:
- one or more chondrocytes produced by the method comprising:
- a) culturing toti-, pluri-, or multi-potent stem cells in a stem cell medium as adherent cells;
- b) pre-differentiating the toti-, pluri-, or multi-potent stem cells into toti-, pluri-, or multi-potent stem cell-derived mesenchymal stem cell (MSC)-like cells in a MSC medium, wherein the method does not result in the formation of embryoid bodies;
- and
- c) differentiating the toti-, pluri-, or multi-potent stem cell-derived MSC-like cells into one or more cells of a mesenchymal lineage.
A method for treating a cartilage disease or disorder, comprising:
- delivering a composition to a joint affected by the cartilage disease or disorder, wherein the composition comprises one or more chondrocytes produced by the method comprising:
- a) culturing the toti-, pluri-, or multi-potent stem cells in stem cell media as adherent cells;
- b) pre-differentiating the toti-, pluri-, or multi-potent stem cells into toti-, pluri-, or multi-potent stem cell-mesenchymal stem cell (MSC)-like cells in MSC medium, wherein the method does not result in the formation of embryoid bodies;
- and
- c) differentiating the toti-, pluri-, or multi-potent stem cell-derived MSC-like cells into one or more cells of a mesenchymal lineage.
Dependencies are inferred from the claim text.