Treatment of collagen defects using protein solutions
AssigneeBiomet Biologics, LLC
InventorsAndrea MATUSKA, Krista O'SHAUGHNESSEY, Jennifer E. WOODELL-MAY
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Abstract
Methods of stimulating collagen production, including stimulation of chondrocyte production, at the site of a defect. Methods include administering to the site of a defect at least two proteins from the group IL-1ra, sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1RII.
Claims28
A method for stimulating chondrocyte production at a site of a collagen defect in a mammalian subject, comprising administering to the site a composition comprising at least two proteins selected from the group consisting of IL-1ra, sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1RII, wherein the concentration of each protein in the composition is greater than the concentration of the protein in normal blood.
A method for stimulating chondrocyte production at a site of a collagen defect in a mammalian subject, comprising administering to the site a blood-derived composition comprising:
- (a) interleukin-1 receptor antagonist (IL-1ra), at a concentration at least 3 times greater than the concentration of IL-1ra in normal blood;
- (b) soluble tissue necrosis factor-r1 (sTNF-r1), at a concentration at least 2 times greater than the concentration of IL-1ra in normal blood;
- (c) cytokine-producing cells at a concentration at least 2 times greater than the concentration of cytokine-producing cells in normal blood;
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- (d) platelets, at a concentration at least 2 times greater than the concentration of platelets in normal blood.
A method for stimulating chondrocyte production at a site of a collagen defect in a mammalian subject, comprising:
- (a) obtaining a cytokine cell suspension from the subject;
- (b) fractionating the liquid to produce an autologous protein solution comprising interleukin-1 receptor antagonist;
- (c) administering the autologous protein solution to the site of the defect in the subject.
Dependencies are inferred from the claim text.