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US 2014/0271571 A1Published application
Cell Culture Cassettes and Incubator
AssigneeSage Science, Inc.
InventorGary Paul Magnant
Life of this patent
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Abstract
The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.
Claims62
A cassette for culturing adherent cells comprising a first film and a second film, wherein
- the first and/or second film comprises at least one sensor.
An incubator comprising:
- a housing;
- a computing device disposed within the housing;
- a chassis that is coupled to the housing and configured to receive a plurality of cassettes for culturing cells, the chassis including a plurality of ports that are coupled to the computing device and configured to interface with corresponding connectors of the cassettes that receive data from sensors disposed within the cassettes;
- a fluidic manifold that houses a media, wherein a conduit of the fluidic manifold includes a plurality of nodes configured to interface with corresponding nodes on cassettes;
- wherein the computing device is configured to
- i) receive a schedule of processes to apply to cell cultures in cassettes;
- ii) receive and store data regarding environmental conditions within the cassettes;
- and
- iii) operate the incubator according to the schedule of processes and received data regarding the environmental conditions.
6 more elements
A method of operating an incubator, the method comprising:
- storing, by a computing device, parameters for a schedule of processes to apply to cell cultures in cassettes;
- monitoring, by the computing device, data about environmental conditions within the cassettes;
- matching, by the computing device, received data about the environment conditions with at least one parameter for a process on the schedule;
- and
- applying, by the computing device, the process to the cell culture within the cassettes.
2 more elements
Dependencies are inferred from the claim text.