Stabilized compositions against ionising radiation
AssigneeMicroarray Limited
InventorsPaul Davis, Jan Jezek, Andrew Austin
Life of this patent
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Abstract
This invention relates to improvements to medical devices such as biosensors containing proteins such as oxidoreductases, for example oxidase and/or peroxidase enzymes. More generally it relates to novel compositions containing proteins which are stabilised to ionising radiation.
Claims24
A medical device comprising an aqueous composition lactate oxidase and two or more protective substances, the first protective substance being methionine or the anion of an organic carboxylic acid not being an amino acid having a rate of reaction with hydroxyl radicals of greater than 109 Lmol −1s−1 and the second protective substance, different to the first protective substance, being selected from the list consisting of aromatic amino acids, nicotinate, purine, methionine and malate and wherein none of the protective substances is lactate.
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A medical device which is a lactate biosensor for use on a wound in the skin of a human or animal, the biosensor being sealed in packaging and comprising a sealed opening which, in use, the opening is exposed and placed over the wound site, the biosensor comprising a sensing means comprising an aqueous composition comprising lactate oxidase enzyme in hydrated condition together with two or more protective substances, the first protective substance being the anion of an organic carboxylic acid not being an amino acid or lactate and having a rate of reaction with hydroxyl radicals of greater than 109 Lmol −1s−1 and the second protective substance, different to the first protective substance, being selected from the list consisting of aromatic amino acids, nicotinate, purine, methionine and malate, and a hydrogen peroxide indicator means, the packaging being in contact with the indicator means and being transparent over a region of contact with the indicator means, the biosensor further comprising a means for preventing the ingress of any molecule larger than lactate into the sensing means, thereby allowing lactate to enter the biosensor from the wound, being oxidised to form hydrogen peroxide by the action of the lactate oxidase, the hydrogen peroxide thereby triggering the indicator means to indicate the presence of lactate in the wound.
Dependencies are inferred from the claim text.